RVFV MP12-Luc IFNAR-/- Mouse Model

Interferon a/b receptor deficient (IFNAR -/-) mice were previously found to be susceptible to the live attenuated vaccine strain of Rift Valley fever virus (RVFV) MP12 [1], providing a BSL-2 model to study RVFV.  In this model mice are challenged with 104 pfu of MP-12-Luc, delivered by intraperitoneal infection. MP12-Luc encodes renilla luciferase in place of the viral protein NSs [2], allowing in vivo monitoring of viral replication using the IVIS Lumina II from Caliper Life Sciences.   MP12-Luc is replication competent and routinely induces uniform mortality in mice 4-5 days post infection at 10^4 pfu.  Animals develop symptoms that are reflective of severe human disease including high viral titers in the liver which can be visualized by a robust luminescence signal in liver.   Additional readouts for this model include weight, survival, health observations, serum and organ titers, and cytokine analysis.

RVFV-MP12+Luc mouse infection_revised

References:

1. Bouloy M et al. Genetic Evidence for an Interferon-Antagonistic Function of Rift Valley Fever Virus Nonstructural Protein NSs. J Virol. 2001 February; 75(3): 1371–1377.

2. Ikegami T et al. Rescue of infectious rift valley fever virus entirely from cDNA, analysis of virus lacking the NSs gene, and expression of a foreign gene. J Virol. 2006 Mar;80(6):2933-40.

Contact information for Kylene Kehn-Hall Ph.D.