A key feature of the BRL’s extensive research capabilities is that they can be used within the BSL-3 spaces for research involving hazardous agents. Samples that cannot be processed within the BSL-3 are treated to inactivate the infectious agents and then handled at BSL-2.
• Molecular Biological Techniques —Accuri C6 Flow Cytometer four-color detection
• Laser Capture Microdissection—Dissection of individual pathogen-infected cells for performing proteomic, genomic, and transcriptomic analysis of tissue sections
• Imaging—Nikon TE-2000 confocal microscope equipped with a live cell imaging system
• Real-Time PCR Instrument—Real-time identification
• Universal Plate Reader—Luminescent, fluorescent, and absorbance-based 96-well assays
Available technological platforms:
- Mass Spectrometry (LC-MS/MS, MALDI-MS, LAESI-MS)
- Reverse Phase protein MicroArray (RPMA) for phospho-proteomics and phospho-signaling
- Multiplexed profiling of chemokine and cytokine mediators
Applications: Vaccine and therapeutic efficacy testing:
- Sample generation and testing using in vitro and in vivo models (challenge studies)
- Correlates of protection, immunogenicity, efficacy
- Mechanism of action (adjuvants)
• Histology – Necropsy suite with downdraft table and hoodline for the collection of animal tissue samples and histological preparation of tissue to include a cryostat for creating tissue sections:
• Nanoparticles – Materials that enhance the discovery and preservation of biomarkers in body fluids for diagnostic, therapeutic, and vaccine targets
• In-vivo imaging — IVIS Lumina II (Caliper Life Sciences) is an in-vivo imaging system that uses a novel patented optical imaging technology to facilitate non-invasive longitudinal monitoring of disease progression and cell trafficking patterns in living animals. The IVIS system is a versatile and advanced in-vivo imaging system.
• Virological Assays – Multiple assays are available for the quantification of viral loads, including standard plaque assays, 96-well plaque assays, absolute q-RT-PCR assays, and focus forming assays.
• Bacterial Assays – Assays available include microbial identification through biochemical assays or 16S rRNA, compound library and combinational library screening, minimal inhibitor concentration (MIC) assays, minimal bactericidal concentration (MRC) assays, time-kill kinetic assays, resistance development/mutant selection and microbial quantitation through q-PCR.