Aarthi Narayanan, Ph.D., Assistant Professor

 

Education 

PhD – University of Georgia, Athens, Georgia

Post doctoral research – Fred Hutchinson Cancer Research Center, Seattle
National Institutes of Health (NIAID, NIH), Maryland

 

Research Interest

  • Host Pathogen interactions: Interaction of viral proteins with host components (mitochondria, kinases) and consequences to viral multiplication.
  • Application of proteomics methodologies to the identification of novel host-based targets for development of therapeutics.
  • Application of hydrogel nanoparticles for characterization of host and pathogen (Dengue) components – development of novel diagnostic methodologies.
  • Proteomics approaches to develop novel metrics for evaluation of vaccine efficacy – correlates of immune responsiveness.

Research Program 

The first major focus of my research is to understand host responses to infection by bunyaviruses such as Rift Valley fever virus (RVFV), alphaviruses such as Venezuelan Equine Encephalitis Virus (VEEV) and human retroviruses such as HIV.  Specifically, I am interested in deciphering the influences of specific phosphosignaling events occurring in infected host cells that correlate with viral replication and in utilizing such events that are crucial for pathogen replication to design novel therapeutic inhibitors.  In addition to identifying novel therapeutic candidates, my research interest also extends to defining the mechanistic basis behind pathogen inhibition when using host-based therapeutics.

The second aspect of my research focus includes extracellular membranous vesicles called exosomes, specifically those produced by virally infected cells (Bunyaviruses, Alphaviruses, Human retroviruses).  Exosomes from infected cells will differ significantly in a proteomic and genomic capacity from those produced by uninfected cells.  Such information can be exploited to understand transfer for genetic and proteomic information to naïve cells (intercellular communication).   Such information can also be utilized to discover novel disease/pathogen specific biomarkers, vaccine and therapeutic candidates.

Selected publications (2012-2014)

1.The role of IKKb in Venezuelan Equine Encephalitis Virus infected cells.  Mamaya A, Voss K, Sampey G, Senina S, De la Fuente C, Mueller C, Kehn-hall K, Kashanchi F, Bailey C, Mogelsvang S, Petricoin E, Narayanan A.  PLoS One. 2014 [accepted].

2.Proteomic strategies for discovery of novel diagnostic and therapeutic targets for infectious diseases.  Mamaya A, Baer A, Voss K, Mueller C, Campbell C, Kehn-Hall K, Bailey C, Petricoin E, Narayanan A.  Pathogens and Disease. 2014 [accepted].

3.Reactive oxygen species activate NFκB (p65) and p53 and induce apoptosis in RVFV infected liver cells. Narayanan A, Amaya M, Voss K, Chung M, Benedict A, Sampey G, Kehn-Hall K, Luchini A, Liotta L, Bailey C, Kumar A, Bavari S, Hakami RM, Kashanchi F.  Virology. 2014 Jan 20;449:270-86.

4.The use of NanoTrap particles as a sample enrichment method to enhance the detection of Rift Valley Fever Virus.  Shafagati N, Narayanan A, Baer A, Fite K, Pinkham C, Bailey C, Kashanchi F, Lepene B, Kehn-Hall K.  PLoS Negl Trop Dis. 2013 Jul 4;7(7):e2296.

5.Exosomesderived from HIV-1-infected cells contain trans-activation response element RNA.  Narayanan A, Iordanskiy S, Das R, Van Duyne R, Santos S, Jaworski E, Guendel I, Sampey G, Dalby E, Iglesias-Ussel M, Popratiloff A, Hakami R, Kehn-Hall K, Young M, Subra C, Gilbert C, Bailey C, Romerio F, Kashanchi F.  J Biol Chem. 2013 Jul 5;288(27):20014-33.

6.Complex role of microRNAs in HTLV-1 infections.  Sampey GC, Van Duyne R, Currer R, Das R, Narayanan A, Kashanchi F. Front Genet. 2012 Dec 17;3:295.

7.Curcumin inhibits Rift Valley fever virus replication in human cells. Narayanan A, Kehn-Hall K, Senina S, Lundberg L, Van Duyne R, Guendel I, Das R, Baer A, Bethel L, Turell M, Hartman AL, Das B, Bailey C, Kashanchi F.  J Biol Chem. 2012 Sep 28;287(40):33198-214.

8.  Modulation of GSK-3β activity in Venezuelan equine encephalitis virus infection.  Kehn-Hall K, Narayanan A, Lundberg L, Sampey G, Pinkham C, Guendel I, Van Duyne R, Senina S, Schultz KL, Stavale E, Aman MJ, Bailey C, Kashanchi F.  PLoS One. 2012;7(4):e34761.

9.  In vivo murine and in vitro M-like cell models of gastrointestinal anthrax.  Tonry JH, Popov SG, Narayanan A, Kashanchi F, Hakami RM, Carpenter C, Bailey C, Chung MC.  Microbes Infect. 2013 Jan;15(1):37-44. doi: 10.1016/j.micinf.2012.10.004.

10. Bacillus anthracis-derived nitric oxide induces protein S-nitrosylation contributing to macrophage death.  Chung MC, Narayanan A, Popova TG, Kashanchi F, Bailey CL, Popov SG. Biochem Biophys Res Commun. 2013 Jan 4;430(1):125-30.

 

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