Edward G. Niles, Ph.D.

Biosketch

Dr. Niles is an emeritus professor of microbiology at the State University of New York at Buffalo School of Medicine.  He received his Ph.D. in biochemistry from the University of Massachusetts, Amherst.  After postdoctoral studies at Yale University, he joined the Biochemistry Department at the SUNY Buffalo. Subsequent to leaving UB, after more than three decades as an academic scientist, Dr. Niles joined BARDA, a division of ASPR in the Department of Health and Human Services.  As an Interdisciplinary Scientist, his efforts focused on the development of drugs and vaccines directed against agents of biodefense interest, principally smallpox virus.

Research Interests

Over his career, research in the Niles laboratory ranged from studies on mechanisms of gene transcription and RNA processing in viral and cellular systems to signal transduction pathways in the helminthes parasite Schistosoma mansoni.  Principle efforts focused on vaccinia virus transcription and mRNA cap formation.  Discovery and development of novel antivirals, antibiotics and vaccines remain topics of keen interest.

Selected Publications

Mohamed, M.R. and Niles, E.G. (2000). Interaction between nucleoside triphosphate phosphohydrolyase I (NPH I) and the H4L subunit of the viral RNA polymerase is required for vaccinia virus early gene transcript release. J. Biol. Chem.275: 25798-25804.

Mohamed, M.R. and Niles, E.G. (2001) The Viral RNA Polymerase H4L Subunit is Required for Vaccinia Virus Early Gene Transcription Termination.  J. Biol. Chem 276: 20758-20765.

Piacente. S.C., Christen, L.A., Mohamed, M.R. and Niles, E.G. (2003) Effect of Selected Mutations in the C-terminal Region of the Vaccinia Virus Nucleoside Triphosphate Phosphohydrolase I (NPH I) on Binding to the H4L Subunit of the Viral RNA Polymerase and Early Gene Transcription Termination, in vitro.  Virology 310: 109-117.

Mohamed, M.R. and Niles, E.G. (2003) UUUUUNU oligonucleotide stimulation of vaccinia virus early gene transcription termination, in trans.  J. Biol. Chem. 278: 11,794-801.

Mohamed, M.R and Niles, E.G. (2003).  UUUUUNU Stimulation of Vaccinia Virus Early Gene Transcription Termination:  Oligonucleotide Sequence and Structural Requirements for Stimulation of Premature Termination, in vitro.  J. Biol. Chem. 278: 39534-39541.

Osman, A., Niles, E.G., LoVerde, P.T. (2004) Expression of functional Schistosoma mansoni Smad4: Role in Erk-mediated transforming growth factor-beta (TGF-b) down regulation. J. Biol. Chem. 279: 6474-6486.

Mohamed, M.R. and Niles, E.G. (2004). UUUUUNU oligonucleotide inhibition of RNA synthesis in vaccinia virus cores.  Virology 324: 493-500.

Wu, W., Niles, E.G., El-Sayed, N., Berriman, M. and Loverde, P.T. (2006). Schistosoma mansoni nuclear receptors: sixteen new members and a novel subfamily.  Gene 366: 303-315.

Mohamed, M.R., Piacente, S.C., Dickerman, B.K. and Niles, E.G. (2006). Effect of UTP Sugar and Base Modifications on Vaccinia Virus Early Gene Transcription  Virology, 349: 359-370.

Piacente, S., Christen, L.A., Dickerman, B., Mohamed, M.R. and Niles, E.G. (2008) Determinants of Vaccinia virus early gene transcription termination. Virology 376: 225-235.

Christen, L.A., Piacente, S., Mohamed, M.R. and Niles, E.G. (2008).  Vaccinia virus early gene transcription termination factors VTF and Rap94 interact with the U9 termination motif in the nascent RNA in the transcription ternary complex.  Virology 376: 211-224.