Rift Valley fever virus (RVFV) is naturally transmitted by mosquito vectors; therefore a subcutaneous model of infection was developed to mimic this route of infection. In this model, female Balb/c mice, 6-8 weeks of age, are challenged by subcutaneous injection with 1000 pfu of RVFV ZH501 which results in 100% mortality. The LD50 of this model was determined to be 50 pfu, which makes our standard dose of 1000 pfu equal to twenty LD50 doses, which is a very stringent model of infection. Animals develop symptoms that are reflective of severe human disease including high viral titers in the liver. Common readouts for this model include weight, survival, health observations, serum and organ titers, and cytokine analysis.